Bioneer AccuRapid Cell-Free Protein Expression Kit
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Recombinant human DAPK1 (1-363) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Death-associated protein kinase 1 (DAPK1) is a positive mediator of apoptosis induced by gamma-interferon. Activation of DAPK occurs via dephosphorylation of Ser-308 and subsequent association of calcium/calmodulin (1). DAPK is rapidly dephosphorylated in response to tumor necrosis factor or ceramide and then subsequently degraded via proteasome activity. The decline in DAPK expression is paralleled with increased caspase activity and cell apoptosis. Studies suggest that the apoptosis regulatory activities mediated by DAPK are controlled both by phosphorylation status and protein stability (2). Kinase Enzyme System contains: Kinase: DAPK1, 10ug (Human, recombinant; amino acids 1-363). MW: ~71kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT, Ca2+/Calmodulin Solution. DAPK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/1612/.
