Bioneer ExiProgen EC1 Protein Synthesis Kit (automated)
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant full-length human CLK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CLK3, also known as CDC-like kinase 3, encodes a serine/threonine-type protein kinase with a non-conserved N-terminal domain. A long and short isoform (phclk3 and pclk3/152) result from alternative splicing and coexist in different tissues (1). The CLK3 protein has the molecular functions of ATP binding, nucleotide binding, protein serine/threonine kinase activity, protein-tyrosine kinase activity and transferase activity, and the CLK3 protein localizes in both the cytoplasm and nuclear compartment. CLK3 is thought to regulate the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Kinase Enzyme System contains: Kinase: CLK3, 10ug (Human, recombinant full-length). MW: ~86kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. CLK3 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/1198/.
