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Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags. AMPK is a heterotrimeric protein kinase consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol. Kinase Enzyme System contains: Kinase: AMPK (A1/B1/G1), 10ug (Human, recombinant full-length). MW: ~68kDa (A1); ~38kDa (B1); ~40kDa (G1). Substrate: SAMStide (HMRSAMSGLHLVKRR); derived from mouse acetyl-Coenzyme A carboxylase alpha (amino acid 73-85). Other: Reaction Buffer, DTT, AMP Solution. AMPK (A1/B1/G1) NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5562/.
