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Recombinant full-length human AKT2 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. AKT2 or Protein Kinase B beta is a serine/threonine kinase that is a member of the AKT family. AKT2 like the other AKT members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis and cell motility. The PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of AKT2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1). Kinase Enzyme System contains: Kinase: AKT2, 10ug (Human, recombinant full-length). MW: ~85kDa. Substrate: Modified AKT Substrate peptide (modified CKRPRAASFAE); based on the N-terminus of GSK3. Other: Reaction Buffer, DTT. AKT2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/208/.
