Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant full-length human SGK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the \"AGC\" kinase subfamily, which includes protein kinases A, G, and C, and its catalytic domain is most similar to protein kinase B (PKB). SGK2, like the other two isoforms SGK1 and SGK3, is stimulated by insulin and insulin-like growth factor-1 (IGF-1), and has been shown to enhance Na(+)/K(+)-ATPase activity in a variety of cells. Kinase Enzyme System contains: SGK2 Kinase, 10ug (Human, recombinant, full-length); MW: ~71kDa. Akt Peptide substrate (1mg/ml), (CKRPRAASFAE); derived from N-terminus of GSK3. 5X Reaction Buffer; DTT, 0.1M.
