Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant full-length human PHKG1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PHKG1 or phosphorylase kinase gamma 1 is a member of the Ser/Thr protein kinase family that encodes a protein with one protein kinase domain and two calmodulin-binding domains. Phosphorylase kinase is a crucial glycogenolytic regulatory enzyme. PHKG1 is the catalytic member of a 16-subunit protein kinase complex that contains equimolar ratios of 4 subunit types known as alpha, beta, gamma and delta. Skeletal muscle contains the highest amount of phosphorylase kinase enzymatic activity, although activity is also observed in liver, cardiac muscle, brain, and several other tissues. Kinase Enzyme System contains: PHKG1 Kinase, 10ug (Human, full-length recombinant). MW: ~70kDa. ZIPtide (1mg/ml), (KKLNRTLSFAEPG). 5X Reaction Buffer, 0.1M DTT.
