Bioneer ExiProgen EC1 Protein Synthesis Kit (automated)
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Recombinant human MLK4 (amino acids 114-420) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK4 or mixed lineage kinase 4 belongs to the superfamily of MAP kinase kinase kinases (MAP3K1) which contain both ser/thr and tyr kinases activity in their catalytic domains. The structure of this kinase family incorporates an N-terminal Src homology (SH3) domain, followed by the kinase domain, a leucine zipper region, and a CDC42 /RAC -interactive binding (CRIB) motif, but divergent C-terminal regions. MLK4 is highly expressed in kidney and pancreas. MLK4 is a negative regulator of TLR4. Kinase Enzyme System contains: MLK4 Kinase, 10ug (Human, recombinant; amino acids 114-420). MW: ~61kDa. Native Swine Myelin Basic Protein (MBP) Substrate, (1mg/ml). 5X Reaction Buffer, 0.1M DTT.
