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Full-length recombinant human CK2 alpha 2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK2?2 or casein kinase II alpha 2 is a member of the CK2 family of Ser/Thr protein kinases. CK2a2 plays a fundamental role in cell function and is involved in DNA replication, regulation of basal and inducible transcription, translation and control of metabolism. CK2?2 prefers utilization of acidic proteins such as caseins as substrates. The CK2a2 holoenzyme is a tetramer composed of an alpha chain, an alpha' and two beta chains. The alpha and alpha' chains contain the catalytic site. Kinase Enzyme System contains: CK2alpha2 Kinase, 10ug (Human, recombinant, full-length); MW: ~64kDa. CK2 Substrate (1mg/ml, RRRADDSDDDDD). 5X Reaction Buffer; DTT, 0.1M.
