Transfection

Transfection of physiologically relevant primary cells or stem cells is typically a very difficult task using traditional transfection methods. Additionally, when using relevant cell lines as model systems, the critical issues are to achieve reproducibly efficient transfection with highlevels of viability while matching throughput capability required at each project phase from proof of concept, through to scale-up and screening-like approaches.

With the electroporation-based Nucleofector™ Technology primary cells and stem cells, as well as cell lines, can be consistently transfected at high efficiency. Developed in 1998, Nucleofection™ has evolved through continuous innovation into a comprehensive portfolio of devicesand kits for many different transfection needs.

	Improved Electroporation using Nucleofector™ Technology Electroporation is a transfection technology based on the momentary creation of small pores in cell membranes by applying an electrical pulse. Lonza's Nucleofector™ Technology is an improved electroporation technology that can help you achieve transfection results never before possible in primary cells, stem cells and hard to transfect cell lines with standard electroporation. In contrast to classicalelectroporation, with Nucleofection high efficiencies can be reached using much lower substrate amounts and with only moderate impact on viability. The comprehensive way in which Nucleofector™ Programs and cell type-specific solutions are developed enables nucleic acid substratesdelivery not only to the cytoplasm, but also through the nuclear membrane and into the nucleus. This allows for high efficiencies up to 99% and makes the transfection success independent from any cell proliferation.
	Nucleofector™ Devices The Nucleofector™ Device delivers unique electrical parameters. The electrical settings are pre-programmed for each optimized cell type and can be selected via the device or PC software. We offer three different device platforms plus an add-on device.
	Nucleofector™ Kits for Primary Cells Over the past decade, Nucleofector™ Technology has become the proven method for transfection of primary cells: Well over 100 protocols for transfection of primary cells available High transfection efficiency paired with high cell viability Maintenance of cell functionality Reduced validation and optimization with specific Nucleofection™ Optimized Protocols for Lonza Primary Cells and Media
	Nucleofector™ Kits for Cell Lines With Nucleofection™ even hard-to-transfect cell lines can be transfected with high efficiency: Efficiencies of up to 90% Cell line transfection data for over 500 different cell lines available Nucleofection™ of 2 x 104 to 2 x 107 cells are feasible within one single device Scale-up without reoptimization - Transferability of protocol conditions from small to larger cell numbers with the new 4D-Nucleofector™ System Easy optimization protocols for transfection for quick and streamlined optimization of virtually any cell line Effortless optimization of a variety of cell lines using the 96-well Shuttle™ Add-on Device
	Nucleofector™ Kit Accessories Nucleofector™ PLUS Supplement Minimize transfection variations caused by donor variance, isolation process or cell culture by using Nucleofector™ PLUS Supplement for cryopreservation of larger cell batches in Nucleofector™ Solution. Specialized media for post Nucleofection™ Culture Our optimized Mouse T Cell Medium is recommended to be used in conjunction with the 4D-Nucleofector™ and 96-well Shuttle™ Kits for transfecting mouse T cells with maximum performance. pmaxCloning™ Vector The pmaxCloning™ Vector promotes strong, constitutive expression of cloned DNA inserts in mammalian cells.